Efficient design and production of glycosylated PEDV spike S1 protein
Eva-Maria Uhlemann, Rinku Patel, Brittany Thivierge, Glenn Hamonic, Wolfgang Köster, Trina Racine, Qiang Liu · 2026
Read Efficient design and production of glycosylated PEDV spike S1 protein by Eva-Maria Uhlemann, Rinku Patel, Brittany Thivierge, Glenn Hamonic, Wolfgang Köster, Trina Racine, Qiang Liu (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13493814.txt.
Description
Eva-Maria Uhlemann and others design and produce a clean, tag-free version of the spike S1 protein from porcine epidemic diarrhea virus. In 2026, the study engineers constructs with a secretion signal and a removable tag, expresses the protein in mammalian cell supernatants, then cleaves the tag during purification. S1 binds cell docks and carries major targets for neutralizing immune proteins.
Characterization covers sugar removal, size, shape, and immune reactivity, with the purified protein recognized by hyper-immune serum in lab tests. Vaccine team and structural biologists gain a well-characterized, tag-free immunogen for a virus that causes up to complete mortality in suckling piglets and heavy losses in pigs.
Catalog record
| CREATOR | Eva-Maria Uhlemann, Rinku Patel, Brittany Thivierge, Glenn Hamonic, Wolfgang Köster, Trina Racine, Qiang Liu |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 40 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |