Optimization of an improved chemically defined medium for high-efficiency expression of glucokinase in Escherichia coli
Yuhan Ma, Jiahao Kong, Zongjin Wang, Sitong Chen, Xu Yang, Min Chen, Zhenguo Wen, JianYe Xia · 2026
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Description
Yuhan Ma and others optimize a chemically defined growth medium for producing glucokinase, an enzyme used in blood glucose testing, in E. coli. In 2026, the study tackles a trade-off: complex media support growth but vary between batches, while simple minimal media are consistent but limit protein yield. High-throughput respiration and growth screening plus machine-learning-guided trials refine the recipe.
The optimized medium raised soluble glucokinase levels thirty-three-fold over the starting recipe, reaching seventy-four percent of complex-media yield at seventy-four percent lower cost. In a 7.5-litre bioreactor it supported dense growth and a titer above 920 milligrams per litre, far exceeding standard media. The recipe offers a cheaper, reproducible route for this and potentially other high-value enzymes.
Catalog record
| CREATOR | Yuhan Ma, Jiahao Kong, Zongjin Wang, Sitong Chen, Xu Yang, Min Chen, Zhenguo Wen, JianYe Xia |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 60 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |