Engineering Komagataella phaffii for secreted and surface-displayed production of SARS-CoV-2 receptor-binding domain and its immunogenicity evaluation
Kostyantyn Dmytruk, Olena Dmytruk, Marta Semkiv, Roksolana Vasylyshyn, Lyubov Fayura, Lidia Gaffke, Magdalena Podlacha, Zuzanna Cyske, Karolina Pierzynowska, Patrick Budylowski, Mario Ostrowski, Grzegorz Wegrzyn, Andriy Sibirny · 2026
Read Engineering Komagataella phaffii for secreted and surface-displayed production of SARS-CoV-2 receptor-binding domain and its immunogenicity evaluation by Kostyantyn Dmytruk, Olena Dmytruk, Marta Semkiv, Roksolana Vasylyshyn, Lyubov Fayura, Lidia Gaffke, Magdalena Podlacha, Zuzanna Cyske, Karolina Pierzynowska, Patrick Budylowski, Mario Ostrowski, Grzegorz Wegrzyn, Andriy Sibirny (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13491813.txt.
Description
Kostyantyn Dmytruk leads a thirteen-author team engineering in 2026 the yeast Komagataella phaffii to produce the receptor-binding domain of the coronavirus that causes COVID-19, a prime vaccine ingredient. Yeast factories are cheap and scalable, and the team built strains that either secrete the protein into the broth or display it on their outer surface like a billboard.
Small linker tweaks lifted secretion to fifty milligrams per liter with little protein trapped inside the cells. Microscopy confirmed surface display in the billboard strain. Mice injected with the purified protein made strong antibody responses, and their blood blocked the virus protein from latching onto its human receptor. Feeding mice the displaying yeast produced comparable results, supporting a whole-cell oral vaccine concept the authors want to develop further.
Catalog record
| CREATOR | Kostyantyn Dmytruk, Olena Dmytruk, Marta Semkiv, Roksolana Vasylyshyn, Lyubov Fayura, Lidia Gaffke, Magdalena Podlacha, Zuzanna Cyske, Karolina Pierzynowska, Patrick Budylowski, Mario Ostrowski, Grzegorz Wegrzyn, Andriy Sibirny |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 33532 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |