Enhanced replication and immunogenicity of a genome-modified non-replicating vaccinia virus Tiantan strain for robust low-dose protection in mice
Jiao Ren, Shiyuan Liu, Hang Yuan, Yiyi Wu, Zhihao Zhao, Yawei Wang, Baoying Huang, Wenling Wang, Houwen Tian, Wenjie Tan · 2026
Read Enhanced replication and immunogenicity of a genome-modified non-replicating vaccinia virus Tiantan strain for robust low-dose protection in mice by Jiao Ren, Shiyuan Liu, Hang Yuan, Yiyi Wu, Zhihao Zhao, Yawei Wang, Baoying Huang, Wenling Wang, Houwen Tian, Wenjie Tan (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13483249.txt.
Description
Ren and colleagues engineered in 2026 a safer vaccinia vaccine strain that grows far better in production cells and protects mice at very low doses. The parent virus cannot copy itself in human cells, a safety plus, but it grew poorly and raised weak immune responses. Two gene edits fixed both flaws.
The edited virus yielded more than six hundred eighty times more virus in production cells than its parent, while harming mice far less than the older vaccine strain. Two shots raised strong antibody levels against both vaccinia and monkeypox, plus a robust cell-based response. Every mouse given either a low two-dose course or one larger dose survived a lethal challenge. The authors present the strain as a next-generation vaccine candidate for mpox control.
Catalog record
| CREATOR | Jiao Ren, Shiyuan Liu, Hang Yuan, Yiyi Wu, Zhihao Zhao, Yawei Wang, Baoying Huang, Wenling Wang, Houwen Tian, Wenjie Tan |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 73 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |