Moderate-temperature DNA cleavage activity of TtAgo activated by dCTP/TthSSB for one-step isothermal microRNAs detection
Jie Fang, Changjing Yuan, Lanlang Peng, Sen Chen, Qinli Pu, Marc Lamy de la Chapelle, Yang Luo, Weiling Fu, Weixian Chen · 2026
Read Moderate-temperature DNA cleavage activity of TtAgo activated by dCTP/TthSSB for one-step isothermal microRNAs detection by Jie Fang, Changjing Yuan, Lanlang Peng, Sen Chen, Qinli Pu, Marc Lamy de la Chapelle, Yang Luo, Weiling Fu, Weixian Chen (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13478391.txt.
Description
Jie Fang and coauthors published a 2026 methods study making a heat-loving DNA-cutting enzyme work at body-friendly temperatures. The enzyme TtAgo normally needs 65 to 85 degrees Celsius, which limits bedside testing. Adding a DNA building block called dCTP plus a protective binding protein from the same bacterium changed that behavior.
The two additives loosened the enzyme's shape and helped it grab target strands, together enabling strong cutting between 37 and 60 degrees. The team built a one-step test for microRNAs, the tiny RNA markers linked to disease, reaching femtomolar sensitivity within 40 minutes. In a 151-sample clinical group, the test separated liver cancer patients from controls as accurately as the standard laboratory method.
Catalog record
| CREATOR | Jie Fang, Changjing Yuan, Lanlang Peng, Sen Chen, Qinli Pu, Marc Lamy de la Chapelle, Yang Luo, Weiling Fu, Weixian Chen |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 93717 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |