Design and transfection of CRISPR/Cas9 constructs for the myostatin gene in Labeo rohita muscle cells
Gowhar Iqbal, Nevil Pinto, Darshan Pawaskar, Arvind A Sonwane, Kiran D Rasal, Lukram Sushil Singh, Nidarshan N Chikkathimmashetty, Mukunda Goswami · 2026
Read Design and transfection of CRISPR/Cas9 constructs for the myostatin gene in Labeo rohita muscle cells by Gowhar Iqbal, Nevil Pinto, Darshan Pawaskar, Arvind A Sonwane, Kiran D Rasal, Lukram Sushil Singh, Nidarshan N Chikkathimmashetty, Mukunda Goswami (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13476449.txt.
Description
Gowhar Iqbal and co-authors build gene-editing tools in 2026 for the myostatin gene in rohu, a major farmed carp. Myostatin brakes muscle growth, so editing it could raise yields. Guide RNAs for the first gene segment were built into a glowing editor plasmid. Sanger reading confirmed the guides sat in the right place and direction.
Muscle cells from the fish glowed at three culture ages, showing the editor entered and switched on each time. Entry held steady from early to late passages of the cell line. The groundwork supports later edits in live fish and eggs. Muscle gene editing in aquaculture gains a tested starter kit.
Catalog record
| CREATOR | Gowhar Iqbal, Nevil Pinto, Darshan Pawaskar, Arvind A Sonwane, Kiran D Rasal, Lukram Sushil Singh, Nidarshan N Chikkathimmashetty, Mukunda Goswami |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 30224 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |