New Insights into Binding of G-segment DNA to the Active Site of Escherichia coli Topoisomerase III
Kemin Tan, Thirunavukkarasu Annamalai, Lucy Stols, Md Anisur Rahman Bhuiyan, Yuk-Ching Tse-Dinh · 2026
Read New Insights into Binding of G-segment DNA to the Active Site of Escherichia coli Topoisomerase III by Kemin Tan, Thirunavukkarasu Annamalai, Lucy Stols, Md Anisur Rahman Bhuiyan, Yuk-Ching Tse-Dinh (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13476441.txt.
Description
An enzyme that untwists DNA must grip its strand just so, and new crystal images show a halfway grip no one had seen. Kemin Tan and co-authors captured a bacterial topoisomerase bound to short single DNA strands. Five structures, each sharp in detail, show the strand held by only one of two grooves. The groove near the cutting site stays closed in this mode. Contacts with the strand mix direct touches and water bridges.
The pictures suggest binding happens in steps, with the first groove engaging early. A new metal-holding site also appeared on a flexible loop near the active center. Such stepwise grip may shape how the enzyme cuts safely. The work refines models of a drug-target enzyme family.
Catalog record
| CREATOR | Kemin Tan, Thirunavukkarasu Annamalai, Lucy Stols, Md Anisur Rahman Bhuiyan, Yuk-Ching Tse-Dinh |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 42 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |