Molecular monitoring and multiplex qPCR-based detection of Cydia pomonella and Grapholita molesta in apple orchards of Kazakhstan
Alibek Makhambetov, Valeriya Kostyukova, Bakyt Dulat, Zarina Dairbekova, Gulnur Zholdasbek, Kairova Gulshariya, Marina Khusnitdinova, Abay Sagitov, Dilyara Gritsenko · 2026
Read Molecular monitoring and multiplex qPCR-based detection of Cydia pomonella and Grapholita molesta in apple orchards of Kazakhstan by Alibek Makhambetov, Valeriya Kostyukova, Bakyt Dulat, Zarina Dairbekova, Gulnur Zholdasbek, Kairova Gulshariya, Marina Khusnitdinova, Abay Sagitov, Dilyara Gritsenko (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13473341.txt.
Description
Makhambetov and colleagues built in 2026 a rapid DNA test distinguishing two moth pests of Kazakh apple orchards. Field surveys in southern and southeastern Kazakhstan collected specimens, identified by form and by a mitochondrial gene barcode. The team then designed a multiplex quantitative PCR test, which detects both species' DNA in one run.
Codling moth made up about eighty-three percent of collected specimens. The barcode reliably separated the two species. The new test showed high specificity, with no false positives from non-target species. It detected the target DNA with full success down to five picograms per reaction, a tiny amount. The authors present the assay as a fast, sensitive tool for plant-health monitoring in orchards. Quicker species identification helps growers time control measures and meet phytosanitary rules for fruit trade.
Catalog record
| CREATOR | Alibek Makhambetov, Valeriya Kostyukova, Bakyt Dulat, Zarina Dairbekova, Gulnur Zholdasbek, Kairova Gulshariya, Marina Khusnitdinova, Abay Sagitov, Dilyara Gritsenko |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 41 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |