Automated in situ microfluidic Random-seq for robust single-nucleus and spatial total RNA profiling of diverse FFPE specimens
Haide Chen, Yu-Sheng Chen, Shunji Zhang, Jiaye Chen, Hao Yang, Tongqiang Fan, Jianfei Zheng, Yuan Liao, Qian Liang, Yongcheng Wang · 2026
Read Automated in situ microfluidic Random-seq for robust single-nucleus and spatial total RNA profiling of diverse FFPE specimens by Haide Chen, Yu-Sheng Chen, Shunji Zhang, Jiaye Chen, Hao Yang, Tongqiang Fan, Jianfei Zheng, Yuan Liao, Qian Liang, Yongcheng Wang (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13470330.txt.
Description
Haide Chen and colleagues present in 2026 a microfluidic platform that profiles RNA inside preserved tissue blocks without removing it first. Hospitals store most tissue as formalin fixed, paraffin embedded blocks, but the fixing process shreds RNA and frustrates standard sequencing. Their system, called imRandom-seq, uses random primers inside tiny fluid channels to capture the full range of transcripts in place.
One workflow serves bulk tissue, single nuclei, and spatial mapping alike. In head to head tests it found more genes than conventional single nucleus sequencing and probe based kits, lost fewer nuclei, and labeled cell types sensibly. It also handled difficult blocks with heavy enzyme activity and badly fragmented RNA. The authors highlight low hands on time and broad tissue compatibility as the practical gains.
Catalog record
| CREATOR | Haide Chen, Yu-Sheng Chen, Shunji Zhang, Jiaye Chen, Hao Yang, Tongqiang Fan, Jianfei Zheng, Yuan Liao, Qian Liang, Yongcheng Wang |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 78120 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |