B cell receptor–based hybridoma enrichment enables discovery of antibodies against intracellular tumor antigens
Iris Dotan, Yuval Raviv, Débora R. Bublik, Jule A. Kortendieck, Shrey Jain, Yifat Glucksam Galnoy, Nechama I. Smorodinsky, Adi Barzel · 2026
Read B cell receptor–based hybridoma enrichment enables discovery of antibodies against intracellular tumor antigens by Iris Dotan, Yuval Raviv, Débora R. Bublik, Jule A. Kortendieck, Shrey Jain, Yifat Glucksam Galnoy, Nechama I. Smorodinsky, Adi Barzel (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13469021.txt.
Description
Dotan and colleagues build a fishing platform for antibodies against targets hidden inside tumour cells. Their 2026 paper addresses a blind spot: antibody discovery has largely ignored intracellular antigens, assuming antibodies cannot usefully reach them. The team combined classic hybridoma generation, fusing B cells into immortal lines, with flow-cytometric enrichment steered by the antigen itself, using tumour B-cell receptors as the bait.
In a mouse breast cancer model engineered to make a glowing intracellular protein, tumour growth triggered a class-switched B-cell response against that hidden antigen. The selected hybridomas kept both surface receptors and secreted antibodies, enabling efficient selection. The resulting monoclonal antibodies recognised native intracellular targets that conventional screens missed. Given system-wide, one antibody slowed growth of early established tumours. The team also demonstrated the approach with human B cells from tonsils and blood, supporting translational use.
Catalog record
| CREATOR | Iris Dotan, Yuval Raviv, Débora R. Bublik, Jule A. Kortendieck, Shrey Jain, Yifat Glucksam Galnoy, Nechama I. Smorodinsky, Adi Barzel |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 54 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |