Establishment of Regeneration Propagation Protocol Based on In Vitro Seeds Development of the Endangered Orchid Species Dendrobium flexicaule
Guoye Guo, Yichen Liu, Manyu Su, Xiaoyi Jing, Pengfei Li, Yicai Zhan, Yuhan Jiang, Yuxi Wu, Xinyuan Hou, Bo Li, Xiaomin Hu, Lunguang Yao · 2026
Read Establishment of Regeneration Propagation Protocol Based on In Vitro Seeds Development of the Endangered Orchid Species Dendrobium flexicaule by Guoye Guo, Yichen Liu, Manyu Su, Xiaoyi Jing, Pengfei Li, Yicai Zhan, Yuhan Jiang, Yuxi Wu, Xinyuan Hou, Bo Li, Xiaomin Hu, Lunguang Yao (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13468468.txt.
Description
Guoye Guo and co-authors work out in 2026 a laboratory propagation protocol for Dendrobium flexicaule, an endangered medicinal orchid whose wild stands have been depleted by over-collection. The species seeds are dust-like, carry no food reserves, and almost never germinate in nature. Starting from mature seeds under sterile conditions, the team systematically searched for the best culture media at each growth stage.
For first germination, a standard MS medium with a small dose of the hormone NAA plus potato homogenate reached a germination rate of 98.33 percent, with robust early plantlets. A second medium mix maximized multiplication and differentiation of the young shoots, at rates of 1.88-fold proliferation and 81.33 percent differentiation. A reliable recipe from seed to plantlet gives conservation programs and growers a way to supply demand without stripping wild cliffs and forests.
Catalog record
| CREATOR | Guoye Guo, Yichen Liu, Manyu Su, Xiaoyi Jing, Pengfei Li, Yicai Zhan, Yuhan Jiang, Yuxi Wu, Xinyuan Hou, Bo Li, Xiaomin Hu, Lunguang Yao |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 43 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |