Next-Generation Sequencing in Colorectal Cancer: Real-World Molecular Profiling and Clinical Correlations
Afonso Cunha, Carolina Robalo, Carolina Lemos, Nuno Jorge Lamas, Marisa Domingues dos Santos · 2026
Read Next-Generation Sequencing in Colorectal Cancer: Real-World Molecular Profiling and Clinical Correlations by Afonso Cunha, Carolina Robalo, Carolina Lemos, Nuno Jorge Lamas, Marisa Domingues dos Santos (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13466781.txt.
Description
Cunha and colleagues report real-world gene testing results for colorectal cancer in this 2026 single-center study. Next-generation sequencing, which reads many genes at once, guides modern cancer care, but how it performs in everyday practice is less clear. The team reviewed 97 patients chosen for routine targeted testing on two sequencing platforms and linked the results to their clinical records.
Ninety-one patients, about 94 percent, carried at least one reportable gene change, with 198 changes found in total. The most often altered genes were TP53 in about 56 percent of patients, KRAS in about 40 percent, PIK3CA, and BRAF. About 19 percent of tumors showed microsatellite instability, a sign of faulty DNA repair. Links between mutations and clinical features were weak, and none survived statistical correction for multiple testing.
Catalog record
| CREATOR | Afonso Cunha, Carolina Robalo, Carolina Lemos, Nuno Jorge Lamas, Marisa Domingues dos Santos |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 50 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |