Comparative Evaluation of Quantitative Real-Time PCR and a Laboratory-Developed Chip-Based Real-Time Digital PCR for JAK2 V617F Allele Burden in Myeloproliferative Neoplasms
Sunggyun Park, Kyoungbo Kim, Jung-Sook Ha · 2026
Read Comparative Evaluation of Quantitative Real-Time PCR and a Laboratory-Developed Chip-Based Real-Time Digital PCR for JAK2 V617F Allele Burden in Myeloproliferative Neoplasms by Sunggyun Park, Kyoungbo Kim, Jung-Sook Ha (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13465721.txt.
Description
Sunggyun Park, Kyoungbo Kim, and Jung-Sook Ha compare two lab methods for measuring how much of a blood sample carries the JAK2 V617F mutation, in a 2026 study. That measurement guides care in a group of blood cancers that lack the Philadelphia chromosome change. Their chip-based digital PCR test, built in the lab, went against a commercial real-time PCR kit.
Both methods ran on leftover DNA from 76 mutation-positive patient samples. Detection limits sat close together, slightly favoring the digital test, which also varied less between repeats, above all at low mutation levels where precision matters most. Straight-line accuracy was strong for both. The authors also checked how mutation levels related to routine blood counts and diagnosis groups.
Catalog record
| CREATOR | Sunggyun Park, Kyoungbo Kim, Jung-Sook Ha |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |