Development of an Assay Involving Loop-Mediated Isothermal Amplification with a Lateral Flow Dipstick for Detection of the vanA Gene in Vancomycin-Resistant Enterococci
Saranthum Phurijaruyangkun, Pongbun Tangjitrungrot, Sawanya Pongparit, Naiyana Wattanakul, Bajaree Jantrapanukorn, Rungnapa Veeramano, Suphitcha Augkarawaritsawong, Khurawan Kumkrong, Thitichot Sirothot, Pornpun Jaratsing, Supatra Areekit, Kankanit Rattanathanawan, Somchai Santiwatanakul, Kantima Choosang, Kosum Chansiri · 2026
Read Development of an Assay Involving Loop-Mediated Isothermal Amplification with a Lateral Flow Dipstick for Detection of the vanA Gene in Vancomycin-Resistant Enterococci by Saranthum Phurijaruyangkun, Pongbun Tangjitrungrot, Sawanya Pongparit, Naiyana Wattanakul, Bajaree Jantrapanukorn, Rungnapa Veeramano, Suphitcha Augkarawaritsawong, Khurawan Kumkrong, Thitichot Sirothot, Pornpun Jaratsing, Supatra Areekit, Kankanit Rattanathanawan, Somchai Santiwatanakul, Kantima Choosang, Kosum Chansiri (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13464955.txt.
Description
Saranthum Phurijaruyangkun leads a team that builds a fast strip test for the vanA gene behind vancomycin resistance in enterococci. These hospital germs shrug off a key last-line drug, and slow gene checks delay the response. Their method joins loop-based DNA copying at one steady heat with a dipstick that shows results by eye.
On one hundred urine checks from clinic patients, the strip caught every true positive the reference PCR method found. Twelve other common bacteria triggered no false signal. Full agreement with the standard method held across the whole run. Speed, simple gear, and a naked-eye read suit the test for ward screening, though larger multi-center checks must come before routine use.
Catalog record
| CREATOR | Saranthum Phurijaruyangkun, Pongbun Tangjitrungrot, Sawanya Pongparit, Naiyana Wattanakul, Bajaree Jantrapanukorn, Rungnapa Veeramano, Suphitcha Augkarawaritsawong, Khurawan Kumkrong, Thitichot Sirothot, Pornpun Jaratsing, Supatra Areekit, Kankanit Rattanathanawan, Somchai Santiwatanakul, Kantima Choosang, Kosum Chansiri |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 34410 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |