Synonymous codon-optimized multiplex qPCR for simultaneous detection of Helicobacter pylori and precise drug resistance profiling in infected individuals
Hui-Lan Li, Ya-Li Fan, Tian Zhong, Bi-Yu Lin, Qin-Jin Wang, Jia Xie, Ze-Lin Zhang, Shu-Ping Nie, Hong-Lei Chen, Chao Wu · 2026
Read Synonymous codon-optimized multiplex qPCR for simultaneous detection of Helicobacter pylori and precise drug resistance profiling in infected individuals by Hui-Lan Li, Ya-Li Fan, Tian Zhong, Bi-Yu Lin, Qin-Jin Wang, Jia Xie, Ze-Lin Zhang, Shu-Ping Nie, Hong-Lei Chen, Chao Wu (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13463915.txt.
Description
Hui-Lan Li leads a team that built a one-tube PCR test for Helicobacter pylori that also reads its drug resistance. Standard gene tests can miss resistant strains when silent letter changes block their probes. The new test folds those silent variants into its probe design from the start.
Probe plans drew on mutation records from 453 isolates and covered nearly all known natural variants. The test sensed the germ at very low copy counts and stayed steady across repeat runs. On 316 stomach tissue checks it matched a store kit closely and matched full gene reading almost fully for both target drugs. Clinics gain a faster way to pick the right cure the first time.
Catalog record
| CREATOR | Hui-Lan Li, Ya-Li Fan, Tian Zhong, Bi-Yu Lin, Qin-Jin Wang, Jia Xie, Ze-Lin Zhang, Shu-Ping Nie, Hong-Lei Chen, Chao Wu |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 43 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |