dSaCas9 enables enhanced transcriptional activation in Nicotiana benthamiana compared to its dSpCas9 ortholog
Aaron M Klepper, Amir Akhgari, Heiko Rischer · 2026
Read dSaCas9 enables enhanced transcriptional activation in Nicotiana benthamiana compared to its dSpCas9 ortholog by Aaron M Klepper, Amir Akhgari, Heiko Rischer (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13455137.txt.
Description
Aaron M Klepper and others showed in 2026 that dead SaCas9 fires genes harder in Nicotiana benthamiana than dead SpCas9. SaCas9 from Staphylococcus aureus beats SpCas9 from Streptococcus pyogenes for plant gene cuts, but gene-fire systems had leaned on SpCas9. The team built a one-plasmid set that set both dead forms side by side.
With Golden Gate cloning, the team joined parts for a wide-use gene-fire set. For the first time, dead SaCas9 fired reporter genes harder by aimed promoters than dead SpCas9 in plants, with both forms sharing one target site for a fair test. The pNOS promoter and a cut-down form showed fine-tune skill. Plant biotech teams gain a stronger gene-fire tool for gene control.
Catalog record
| CREATOR | Aaron M Klepper, Amir Akhgari, Heiko Rischer |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 40 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |