Enhanced efficiency in mitotane therapeutic drug monitoring: optimization of chromatographic resolution and column longevity
Anna Sylvia Ferrari Marques, Berenice Bilharinho Mendonca, Helena Panteliou Lima-Valassi · 2026
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Description
Anna Sylvia Ferrari Marques and colleagues write in 2026 to update a laboratory method for monitoring mitotane, a drug for adrenal cancer whose blood levels must be tracked closely. Their earlier liquid chromatography method worked, but its internal standard, a close chemical cousin of the drug, separated poorly from it as the column aged, blurring peaks and risking wrong results. The column itself also clogged, ran at high pressure, and wore out fast under continuous use.
The letter proposes swapping the standard to 2,4-prime-DDT, which extracts and behaves like mitotane yet resolves cleanly from the drug and from plasma components. It also moves the separation onto a different manufacturer's C18 column that promises speed and durability. Because bonding chemistry differs between makers, the team re-optimized flow and temperature while holding the mobile phase fixed. The goal is a faster, sturdier assay that hospital laboratories can run day after day.
Catalog record
| CREATOR | Anna Sylvia Ferrari Marques, Berenice Bilharinho Mendonca, Helena Panteliou Lima-Valassi |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 6 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |