Detection and colocalization of STEC genetic markers in wheat flour using whole cell digital droplet PCR
Sophie Butot, Caroline Gaille, Lise Michot, Mathieu Seppey, Caroline Barretto, Solenn Pruvost, Sophie Zuber · 2026
Read Detection and colocalization of STEC genetic markers in wheat flour using whole cell digital droplet PCR by Sophie Butot, Caroline Gaille, Lise Michot, Mathieu Seppey, Caroline Barretto, Solenn Pruvost, Sophie Zuber (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13447292.txt.
Description
Sophie Butot leads a team testing a droplet-based DNA method to find harmful E. coli in wheat flour in 2026 food safety work. Certain strains make Shiga toxins and can cause severe illness, yet flour is a hard material to test and rules differ by country. Finding several danger genes inside the same cell matters, since scattered genes may come from harmless strains.
Whole cells were split into droplets so gene signals could be checked for co-location in single cells. A new generic E. coli marker was also validated against target and non-target species. Detection limits matched a standard DNA test at under one cell per gram under study conditions. Spiked flour and naturally contaminated shop flour both gave readable patterns, supporting the approach as a flexible base for sharper flour testing.
Catalog record
| CREATOR | Sophie Butot, Caroline Gaille, Lise Michot, Mathieu Seppey, Caroline Barretto, Solenn Pruvost, Sophie Zuber |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 49165 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |