An effective method for isolation and regeneration of Solanum tuberosum mesophyll protoplasts for transgene-free genome editing
Nehanjali Parmar, Shruti Pathania, Vanita Patial, Neha Kaushal, Aditi, Jyoti Verma, Rasna Devi, Dharmendra Kumar, Hemant B. Kardile, Brajesh Singh, Som Dutt · 2026
Read An effective method for isolation and regeneration of Solanum tuberosum mesophyll protoplasts for transgene-free genome editing by Nehanjali Parmar, Shruti Pathania, Vanita Patial, Neha Kaushal, Aditi, Jyoti Verma, Rasna Devi, Dharmendra Kumar, Hemant B. Kardile, Brajesh Singh, Som Dutt (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13446043.txt.
Description
Nehanjali Parmar and colleagues refine a recipe for freeing living cells from potato leaves and growing them back into whole plants, in a 2026 methods paper. Bare cells, called protoplasts, are the doorway to gene editing that leaves no foreign DNA behind. Potato breeding is slow and tangled, so a sturdy cell-to-plant path has wide value.
The team tuned dark pretreatment, a salt pre-soak, enzyme doses, and sugar levels that keep cells plump. A mix of two enzymes over sixteen hours gave the best yield of live cells. A set chain of growth media then carried cells through division, tiny callus growth, shoots, and roots, with young plants hardened in a greenhouse at the end. The path readies potato cells for clean editing.
Catalog record
| CREATOR | Nehanjali Parmar, Shruti Pathania, Vanita Patial, Neha Kaushal, Aditi, Jyoti Verma, Rasna Devi, Dharmendra Kumar, Hemant B. Kardile, Brajesh Singh, Som Dutt |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 45 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |