Measuring the efficiency of a solids-based wastewater extraction using BCoV and validation of a BCoV and PMMoV RT-qPCR duplex assay
Codey E Dueck, Nataliya Santiago, Nikho A Hizon, Ravinder Lidder, Dave Spreitzer, Umar P Mohammed, MacKenzie Dyck, Chand S Mangat, Edgard M Mejia · 2026
Read Measuring the efficiency of a solids-based wastewater extraction using BCoV and validation of a BCoV and PMMoV RT-qPCR duplex assay by Codey E Dueck, Nataliya Santiago, Nikho A Hizon, Ravinder Lidder, Dave Spreitzer, Umar P Mohammed, MacKenzie Dyck, Chand S Mangat, Edgard M Mejia (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13446042.txt.
Description
Codey E. Dueck and colleagues set out a method to grade how well a solids-based extraction pulls viral material from wastewater, in a 2026 paper for surveillance labs. Wastewater tracking of disease leans on extraction, yet each lab needs a yardstick for its own losses. A cattle coronavirus, spiked into samples on purpose, serves as the stand-in.
Heat-based release measured the starting stock more truly than a machine method. The team also found the cattle virus already present in wastewater at low levels, a background any grade must subtract. Sixteen hours of soaking gave the best recovery in their runs. A paired test that reads the cattle virus and a pepper virus at once matched the single tests, which saves time in routine checks.
Catalog record
| CREATOR | Codey E Dueck, Nataliya Santiago, Nikho A Hizon, Ravinder Lidder, Dave Spreitzer, Umar P Mohammed, MacKenzie Dyck, Chand S Mangat, Edgard M Mejia |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 80300 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |