Fluorouridine labeling enables spatiotemporal mapping of transcriptomes and enhancers in vivo
Yuxuan Feng, Yiwei Zhang, Heyuan Lian, Zhenning Wang, Jieming Wang, Li Wu, Charles J David, Fei Xavier Chen, Harihar Basnet · 2026
Read Fluorouridine labeling enables spatiotemporal mapping of transcriptomes and enhancers in vivo by Yuxuan Feng, Yiwei Zhang, Heyuan Lian, Zhenning Wang, Jieming Wang, Li Wu, Charles J David, Fei Xavier Chen, Harihar Basnet (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13444121.txt.
Description
Yuxuan Feng and colleagues build a live-animal RNA tagging method, 5FU-seq, in a 2026 paper. A tagged feed base, 5-fluorouridine, slips into fresh RNA, which lets teams catch gene activity as it happens. Past tags worked mostly in dishes, with weaker uptake and reach.
Their tag entered RNA at higher rates and caught a broader span of messages with finer feel. In mouse brains it exposed stimulus-tied gene webs and a dynamic Nrf2 program during anesthesia-linked sleep loss. A bred mouse line adds cell-type tags in place, so gene maps and enhancer maps come from the same tissue. Checks against sorted-cell RNA and open-chromatin reads held up. The authors offer the kit for tissue-wide time maps.
Catalog record
| CREATOR | Yuxuan Feng, Yiwei Zhang, Heyuan Lian, Zhenning Wang, Jieming Wang, Li Wu, Charles J David, Fei Xavier Chen, Harihar Basnet |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 53936 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |