Guide RNA reprogramming facilitates minimized tracrRNA-dependent off-target and versatile CRISPR/Cas9 engineering
Wenxia Yu, Jun Chen, Junfan Guo, Fang Yu, Ge Wang, Jianxiang Lin, Xiangping Dai, Xinyi Tan, Peixiang Ma, Ligang Wu, Yu Zhang, Shisheng Huang, Pengfei Lan, Qian Bian, Xingxu Huang, Jiao Wei, Tianlin Cheng, Xiaoguo Zheng, Yunbo Qiao · 2026
Read Guide RNA reprogramming facilitates minimized tracrRNA-dependent off-target and versatile CRISPR/Cas9 engineering by Wenxia Yu, Jun Chen, Junfan Guo, Fang Yu, Ge Wang, Jianxiang Lin, Xiangping Dai, Xinyi Tan, Peixiang Ma, Ligang Wu, Yu Zhang, Shisheng Huang, Pengfei Lan, Qian Bian, Xingxu Huang, Jiao Wei, Tianlin Cheng, Xiaoguo Zheng, Yunbo Qiao (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13434305.txt.
Description
Wenxia Yu and a large team report in 2026 that the guide RNA used in CRISPR editing can be split and rebuilt. That same trait lets stray cell RNAs hijack the guide and cause rare off target edits. Machine learning on large guide screens gave rules to cut that risk.
New guides were made to clash with human and mouse RNA so hijack odds fell. Split guides also let one system edit up to six genes at once and read out gene messages. A rebuilt scaffold curbed self editing by the Cas9 enzyme. Safer guides widen what gene editing can do in stem cells.
Catalog record
| CREATOR | Wenxia Yu, Jun Chen, Junfan Guo, Fang Yu, Ge Wang, Jianxiang Lin, Xiangping Dai, Xinyi Tan, Peixiang Ma, Ligang Wu, Yu Zhang, Shisheng Huang, Pengfei Lan, Qian Bian, Xingxu Huang, Jiao Wei, Tianlin Cheng, Xiaoguo Zheng, Yunbo Qiao |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 98224 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |