Read-Level Error Characterization of Rolling-Circle Amplification-Based Nanopore Sequencing of the Circular DNA Virome
Florencia Martino, Kakhangchung Panmei, Dylan Duchen, David L. Thomas, Abraham J. Kandathil, Steven J. Clipman · 2026
Read Read-Level Error Characterization of Rolling-Circle Amplification-Based Nanopore Sequencing of the Circular DNA Virome by Florencia Martino, Kakhangchung Panmei, Dylan Duchen, David L. Thomas, Abraham J. Kandathil, Steven J. Clipman (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13431522.txt.
Description
Florencia Martino and co-authors measured read-level errors in 2026 for a portable gene reading method used on small round viral genomes. The method first copies round DNA with a rolling method that can add its own noise. Pinning down that noise matters when many kin viruses mix in one sample at uneven depth.
The team spiked a known round phage genome into plasma DNA that held target viruses, at set doses. Custom tools counted swaps, extra bases, and lost bases in each read. Error rates sat near two per hundred bases across four test sets. Full rebuild of the known genome worked once the dose passed a floor level, and joint reads matched the true genome in full at modest depth. Below the floor, recovery turned patchy.
Catalog record
| CREATOR | Florencia Martino, Kakhangchung Panmei, Dylan Duchen, David L. Thomas, Abraham J. Kandathil, Steven J. Clipman |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 25663 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |