Protein Extraction From Mycobacterium spp. for Identification by MALDI‐TOF Mass Spectrometry
Lucas Evangelista Marques, Stephanie Chrystine Balestro Mota, Christoffel Opperman, Robin Mark Warren, Emilyn Costa Conceição, Cristina Viana‐Niero · 2026
Read Protein Extraction From Mycobacterium spp. for Identification by MALDI‐TOF Mass Spectrometry by Lucas Evangelista Marques, Stephanie Chrystine Balestro Mota, Christoffel Opperman, Robin Mark Warren, Emilyn Costa Conceição, Cristina Viana‐Niero (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13422670.txt.
Description
Lucas Evangelista Marques and co-authors present a laboratory protocol for extracting proteins from Mycobacterium species so the bacteria can be picked out by MALDI-TOF mass spectrometry. This identification method reads a protein fingerprint, but it only works when proteins are extracted well. Their 2026 protocol targets species-level identification, which is hard in this diverse genus.
The procedure combines heat inactivation, repeated washing of the bacterial mass, mechanical disruption with zirconia beads, and extraction with acetonitrile and formic acid. Troubleshooting and quality control steps are included, and the workflow is designed for Bruker MALDI Biotyper systems. Safety receives special emphasis: the steps meet biosafety level 2 and 3 requirements and limit aerosol generation when handling dangerous strains. The authors aim to standardise preparation across diagnostic and research laboratories.
Catalog record
| CREATOR | Lucas Evangelista Marques, Stephanie Chrystine Balestro Mota, Christoffel Opperman, Robin Mark Warren, Emilyn Costa Conceição, Cristina Viana‐Niero |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 51 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |