Structure of the pre-initiation complex explains CMGE biogenesis
Thomas Pühringer, Berta Canal, Giacomo Palm, Agata Butryn, Emma C. Couves, Oliver Willhoft, Jacob S. Lewis, John F. X. Diffley, Alessandro Costa · 2026
Read Structure of the pre-initiation complex explains CMGE biogenesis by Thomas Pühringer, Berta Canal, Giacomo Palm, Agata Butryn, Emma C. Couves, Oliver Willhoft, Jacob S. Lewis, John F. X. Diffley, Alessandro Costa (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13421386.txt.
Description
Thomas Pühringer and eight colleagues reveal, in a 2026 structural study, how the cell assembles the machine that starts DNA replication. When a cell enters S phase, three activator proteins join a ring-shaped motor loaded on DNA to form two CMGE helicases, which then unwind the double helix in opposite directions.
The team rebuilt the pre-initiation complex from purified yeast proteins and solved its structure with cryo-electron microscopy, catching the firing factors mid-assembly. The structure shows how stepwise binding reshapes the motor for DNA opening and how ATP triggers factor release. One factor, Sld2, both recruits a partner and helps split the paired helicases, expelling the lagging strand. The authors link the mechanism to human replication disorders.
Catalog record
| CREATOR | Thomas Pühringer, Berta Canal, Giacomo Palm, Agata Butryn, Emma C. Couves, Oliver Willhoft, Jacob S. Lewis, John F. X. Diffley, Alessandro Costa |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 87611 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |