High-throughput bead-based cytokine profiling reveals dynamic immune signatures and identifies candidate exploratory biomarkers in CAR-T therapy
Keyuan Pu, Wanying He, Jiayi Feng, Hongyan Li, Chun-Lung Chan, Thomas S. Y. Chan, Eric Tse, Hongzhe Sun · 2026
Read High-throughput bead-based cytokine profiling reveals dynamic immune signatures and identifies candidate exploratory biomarkers in CAR-T therapy by Keyuan Pu, Wanying He, Jiayi Feng, Hongyan Li, Chun-Lung Chan, Thomas S. Y. Chan, Eric Tse, Hongzhe Sun (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13417598.txt.
Description
Pu and colleagues built in 2026 a fast bead system for tracking immune signals during CAR-T therapy. CAR-T therapy uses a patient's own immune cells, re-engineered to attack cancer, but it can trigger a dangerous cytokine storm. Their method makes coded beads in a 96-well plate, raising output from a few bead types a day to 96 types in eight hours.
The team profiled 136 blood samples from 32 patients with large B-cell lymphoma treated with CD19 CAR-T cells. Cytokine patterns over time differed clearly between patients with mild and severe cytokine release syndrome. One signal, TGF-β1 measured before treatment, was linked to later severe syndrome and to worse event-free survival. The authors present baseline TGF-β1 as a candidate warning marker and the bead system as a practical tool for immune monitoring.
Catalog record
| CREATOR | Keyuan Pu, Wanying He, Jiayi Feng, Hongyan Li, Chun-Lung Chan, Thomas S. Y. Chan, Eric Tse, Hongzhe Sun |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 43 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |