A systematic stepwise optimization framework for rapid multi-analyte UPLC–MS/MS plasma analysis with integrated sustainability assessment
Amira E. Abd-Elnabi, Amr M. Mahmoud, Dina A. El Mously, Omnia A. El-Naem · 2026
Read A systematic stepwise optimization framework for rapid multi-analyte UPLC–MS/MS plasma analysis with integrated sustainability assessment by Amira E. Abd-Elnabi, Amr M. Mahmoud, Dina A. El Mously, Omnia A. El-Naem (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13415790.txt.
Description
Amira E. Abd-Elnabi and co-authors propose a six step framework for building fast multi-drug plasma assays, in 2026. Their method couples liquid chromatography with tandem mass spectrometry. Steps run from profiling each drug's chemistry through extraction, column tuning, mobile phase choice, validation, and a greenness score.
The framework was proven on three drugs at once in spiked human plasma: rifaximin, ciprofloxacin, and fluconazole, with ibuprofen as internal standard. Liquid extraction with dichloromethane recovered the drugs well. A simple methanol and water mix separated them quickly on a standard column. Linearity was excellent, and two scoring tools rated the method's environmental footprint.
Catalog record
| CREATOR | Amira E. Abd-Elnabi, Amr M. Mahmoud, Dina A. El Mously, Omnia A. El-Naem |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 44 KB |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |