Limited Impact of Column Chemistry and Length on Proteome Coverage Under High-Speed DIA
Alicia-Sophie Schebesta, Kathrin Korff, Ericka C.M. Itang, Vincent Albrecht, Philipp E. Geyer, Johannes B. Mueller-Reif · 2026
Read Limited Impact of Column Chemistry and Length on Proteome Coverage Under High-Speed DIA by Alicia-Sophie Schebesta, Kathrin Korff, Ericka C.M. Itang, Vincent Albrecht, Philipp E. Geyer, Johannes B. Mueller-Reif (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13400878.txt.
Description
Alicia-Sophie Schebesta and colleagues test, in 2026, whether the chemistry and length of separation columns still matter for proteome depth on the fastest mass spectrometers. Protein detection once depended heavily on good separation. Modern instruments sample so quickly that the balance may have shifted.
The team ran five column chemistries across eight lengths on a high-speed instrument. Despite differing materials and sizes, results converged. The standard chemistries all found over 150,000 precursors and roughly nine thousand protein groups. Retention patterns still differed by chemistry. Yet those differences did not turn into real coverage gains. Column choice, within modern ranges, appears far less critical than before.
Catalog record
| CREATOR | Alicia-Sophie Schebesta, Kathrin Korff, Ericka C.M. Itang, Vincent Albrecht, Philipp E. Geyer, Johannes B. Mueller-Reif |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 62215 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |