ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy development
Merijn Braams, Martijn Cordes, Sandra A. Vloemans, Bas de Mooij, Sandra de Bruin-Versteeg, Ashley Wachtmeester, Anton W. Langerak, Karin Pike-Overzet, Frank J.T. Staal, Kirsten Canté-Barrett, Sander de Kivit · 2026
Read ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy development by Merijn Braams, Martijn Cordes, Sandra A. Vloemans, Bas de Mooij, Sandra de Bruin-Versteeg, Ashley Wachtmeester, Anton W. Langerak, Karin Pike-Overzet, Frank J.T. Staal, Kirsten Canté-Barrett, Sander de Kivit (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13400188.txt.
Description
Merijn Braams and colleagues introduce ABO, a three-dimensional culture system that carries human blood stem cells through the complete development of B cells, in a 2026 study. ABO aggregates human hematopoietic stem and progenitor cells with mouse bone marrow stromal cells. The structure shepherds the cells through pro-B, pre-B, immature, and transitional stages in order.
The decisive test was disease modeling. Stem cells from a patient with RAG1-deficient severe combined immunodeficiency reproduced their developmental arrest inside ABO, and a RAG1 gene therapy lentiviral vector corrected it in the dish. The authors offer the platform as a clinically relevant bench for diagnosing B cell immunodeficiencies and for pre-screening gene therapy products before they reach patients.
Catalog record
| CREATOR | Merijn Braams, Martijn Cordes, Sandra A. Vloemans, Bas de Mooij, Sandra de Bruin-Versteeg, Ashley Wachtmeester, Anton W. Langerak, Karin Pike-Overzet, Frank J.T. Staal, Kirsten Canté-Barrett, Sander de Kivit |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 104712 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |