Development and validation of a recombinant Omp19+31-based indirect hemagglutination assay for serological diagnosis of bovine and ovine brucellosis
Aitbay Bulashev, Alfiya Syzdykova, Anara Kukayeva, Aibek Zhumalin, Guldarigash Kaukabaeva, Mikail Mikailov, Maxat Berdikulov, Bakytkali Ingirbay · 2026
Read Development and validation of a recombinant Omp19+31-based indirect hemagglutination assay for serological diagnosis of bovine and ovine brucellosis by Aitbay Bulashev, Alfiya Syzdykova, Anara Kukayeva, Aibek Zhumalin, Guldarigash Kaukabaeva, Mikail Mikailov, Maxat Berdikulov, Bakytkali Ingirbay (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13392659.txt.
Description
Aitbay Bulashev and colleagues built a new blood test for brucellosis in cattle and sheep using a fused protein stitched from two Brucella surface proteins. Older tests lean on a sugary coating molecule that cross-reacts with other germs and triggers false alarms and needless culling. Their test coats sheep red blood cells with lab-made proteins and watches for clumping, a cheap method needing little equipment.
Fourteen hundred forty serum samples from sick and healthy herds were run through the new test alongside two standard methods. The fused-protein version caught nearly all true cases while producing no false positives in the panel, giving the best overall accuracy of the antigens tried. In a small group of slaughtered positive cattle it flagged every case, including some a commercial kit missed. The team presents it as a sharper tool for herd screening.
Catalog record
| CREATOR | Aitbay Bulashev, Alfiya Syzdykova, Anara Kukayeva, Aibek Zhumalin, Guldarigash Kaukabaeva, Mikail Mikailov, Maxat Berdikulov, Bakytkali Ingirbay |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 72581 |
| LICENSE | https://creativecommons.org/publicdomain/zero/1.0 |
| RIGHTS | CC0 |
| SOURCE | Europe PMC |