Selection of two pronuclei zygotes before electroporation does not improve gene editing efficiency or reduce mosaicism in porcine embryos despite optimized timing and Hoechst staining
Koki Takebayashi, Takeshige Otoi, Manita Wittayarat, Oky Setyo Widodo, Theerawat Tharasanit, Kaywalee Chatdarong, Yuichiro Nakayama, Zhao Namula, Megumi Nagahara, Maki Hirata, Fuminori Tanihara · 2026
Read Selection of two pronuclei zygotes before electroporation does not improve gene editing efficiency or reduce mosaicism in porcine embryos despite optimized timing and Hoechst staining by Koki Takebayashi, Takeshige Otoi, Manita Wittayarat, Oky Setyo Widodo, Theerawat Tharasanit, Kaywalee Chatdarong, Yuichiro Nakayama, Zhao Namula, Megumi Nagahara, Maki Hirata, Fuminori Tanihara (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13392619.txt.
Description
Koki Takebayashi and a large team asked whether picking pig zygotes that clearly show two pronuclei, the paired structures marking normal fertilisation, before electric-pulse gene editing would cut mosaic results. Mosaic embryos carry a mix of edited and unedited cells, which muddies breeding work. Their plan also tuned when to look and how to stain the structures for viewing.
Tracking showed two-pronuclei zygotes peaked at twenty hours after fertilisation, and one gentle staining recipe gave the best later development. Yet edited embryos from picked zygotes formed blastocysts at rates no better than unpicked controls, and mutation rates and mosaic shares matched as well. The negative result matters for labs weighing the extra handling step: on this evidence, selection adds work without improving editing.
Catalog record
| CREATOR | Koki Takebayashi, Takeshige Otoi, Manita Wittayarat, Oky Setyo Widodo, Theerawat Tharasanit, Kaywalee Chatdarong, Yuichiro Nakayama, Zhao Namula, Megumi Nagahara, Maki Hirata, Fuminori Tanihara |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 43476 |
| LICENSE | https://creativecommons.org/publicdomain/zero/1.0 |
| RIGHTS | CC0 |
| SOURCE | Europe PMC |