A versatile dual-color bacterial reporter system highlights two distinct Pseudomonas aeruginosa type 3 secretion system intracellular populations
Christopher J. Corcoran, David G. Glanville, Zachary J. Resko, Erin K. Cassin, Andrew D. Marten, Derrick L. Kamp, Karen L. Visick, Spencer V. Nyholm, Boo Shan Tseng, Abby R. Kroken, Andrew T. Ulijasz · 2026
Read A versatile dual-color bacterial reporter system highlights two distinct Pseudomonas aeruginosa type 3 secretion system intracellular populations by Christopher J. Corcoran, David G. Glanville, Zachary J. Resko, Erin K. Cassin, Andrew D. Marten, Derrick L. Kamp, Karen L. Visick, Spencer V. Nyholm, Boo Shan Tseng, Abby R. Kroken, Andrew T. Ulijasz (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13390426.txt.
Description
Christopher J. Corcoran's team built a dual-colour germ tracker, pCG-VmS, that tracks Pseudomonas aeruginosa's type three release system inside host cells over time. The DNA ring pairs steady green glow with shifting red glow, also allowing protein-placing fusions, for use in dishes, tissues, and whole animals.
In a human eye-surface cell line, the tracker showed a distinct inside-cell sleeping group in which the release system never switches on, alongside an active group. An ex vivo mouse eye infection model proved both readout profiles in tissue, giving teams a space-time tool fitted to other Gram-negative germs.
Catalog record
| CREATOR | Christopher J. Corcoran, David G. Glanville, Zachary J. Resko, Erin K. Cassin, Andrew D. Marten, Derrick L. Kamp, Karen L. Visick, Spencer V. Nyholm, Boo Shan Tseng, Abby R. Kroken, Andrew T. Ulijasz |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 56 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |