Detecting protein higher-order structural changes using kinase as a phospho-labeler
Asato Maeda, Kosuke Ogata, Yasushi Ishihama · 2026
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Description
Asato Maeda's team developed a proteome-wide way to spot protein shape changes by using a kinase as a labelling tool. Their method pairs residue-specific phosphate labelling, which depends on kinase recognition of exposed sites, with quantitative phosphoproteomics after enrichment.
Tests on myoglobin with and without heat denaturation and on cell extracts with and without protease treatment showed labelling efficiency tracking structural state. Comparing extracts before and after RNA digestion then showed proteome-wide shape changes in intact cell material, giving teams a site-resolved readout of protein dynamics inside cells.
Catalog record
| CREATOR | Asato Maeda, Kosuke Ogata, Yasushi Ishihama |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 61 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |