Fluorogenic Coupled Assays Reveal Catalytic Properties, Inhibition Constants and Cellular Location of Mucin‐Active Carbohydrate Sulfatases
Charles W E Tomlinson, Madouc D Bergers, David N Bolam, Ana S Luis, Alan Cartmell, Zachary Armstrong · 2026
Read Fluorogenic Coupled Assays Reveal Catalytic Properties, Inhibition Constants and Cellular Location of Mucin‐Active Carbohydrate Sulfatases by Charles W E Tomlinson, Madouc D Bergers, David N Bolam, Ana S Luis, Alan Cartmell, Zachary Armstrong (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13383068.txt.
Description
Sugars coated with sulfate groups shape health in the gut and beyond, and enzymes that strip the sulfate off are hard to study for lack of good tools. Charles Tomlinson and co-authors build such tools in this 2026 paper: glowing sugar probes that light up only after these enzymes act.
By pairing the probes with a second enzyme that cuts only stripped sugars, the team could measure activity, selectivity, and blocking strength in simple plate tests. The assays worked on purified enzymes and on cell extracts, and they even revealed where inside the cell two gut enzymes sit. The kit should speed the hunt for new enzymes and blockers.
Catalog record
| CREATOR | Charles W E Tomlinson, Madouc D Bergers, David N Bolam, Ana S Luis, Alan Cartmell, Zachary Armstrong |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 35 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |