Optimized iCIEF Fractionation Enables Deep Profiling of Antibody Drug Conjugate Charge Heterogeneity
Eunju Jang, Qunying Zhang, Paul M. Thomas · 2026
Read Optimized iCIEF Fractionation Enables Deep Profiling of Antibody Drug Conjugate Charge Heterogeneity by Eunju Jang, Qunying Zhang, Paul M. Thomas (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13378279.txt.
Description
Eunju Jang and others tune a splitting method for deep mapping of that protein-drug linked drug charge variants in a 2026 study. That protein-drug linked drugs combine antibody aim with cell-killing potency, and charge variants are key quality features to monitor through making. Imaged tiny tube charge sorting is a gold standard for charge analysis, yet comprehensive characterisation needs splitting for mass spectrometry.
Fractionation conditions were tuned to stabilise mobilisation after separation. Collected fractions checked by native size-exclusion mass spectrometry revealed species previously undetectable. Further mass analysis mapped major post-translational modifications and drug load distributions for light and heavy chains. The method gives developers a deeper view of linked drug heterogeneity and can be extended to other complex biomolecules.
Catalog record
| CREATOR | Eunju Jang, Qunying Zhang, Paul M. Thomas |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 28 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |