Simultaneous orthogonal cell engineering by a single CRISPR-Cas9 polyfunctional editor
Deborah Cipria, Tania Baccega, Miriana Rizzo, Piergiuseppe Quarato, Alice Reschigna, Rita El Khoury, Martino Alfredo Cappelluti, Sandra Ammann, Valeria Mollica Poeta, Matteo Conti, Sara Valsoni, Pietro Spinelli, Ivan Merelli, Toni Cathomen, Monica Casucci, Angelo Lombardo · 2026
Read Simultaneous orthogonal cell engineering by a single CRISPR-Cas9 polyfunctional editor by Deborah Cipria, Tania Baccega, Miriana Rizzo, Piergiuseppe Quarato, Alice Reschigna, Rita El Khoury, Martino Alfredo Cappelluti, Sandra Ammann, Valeria Mollica Poeta, Matteo Conti, Sara Valsoni, Pietro Spinelli, Ivan Merelli, Toni Cathomen, Monica Casucci, Angelo Lombardo (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13376164.txt.
Description
Deborah Cipria and co-authors edit T cells in several directions at once with a single CRISPR tool. Their 2026 paper joins gene knockout, gene insertion, and epigenetic silencing in primary human T cells. Multi-break editing normally risks chromosome swaps between cut sites.
Their editor carries an active Cas9 whose cutting is selectively disarmed at silencing targets by shortened guide RNAs. No reciprocal translocations appear. Showcases include swapping the natural T cell receptor for a tumor-selective one and inserting a CAR construct at two kinds of loci. Clinically relevant genes are silenced in multiplex, durably. The authors pitch polyfunctional editing as a safer, wider frame for cancer cell therapy engineering.
Catalog record
| CREATOR | Deborah Cipria, Tania Baccega, Miriana Rizzo, Piergiuseppe Quarato, Alice Reschigna, Rita El Khoury, Martino Alfredo Cappelluti, Sandra Ammann, Valeria Mollica Poeta, Matteo Conti, Sara Valsoni, Pietro Spinelli, Ivan Merelli, Toni Cathomen, Monica Casucci, Angelo Lombardo |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 81 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |