Comparative evaluation of four reference genes for qRT-PCR assays in snow trout (Schizothorax richardsonii) under multiple experimental conditions
Manchi Rajesh, Biju Sam Kamalam, Rini Joshi, Ciji Alexander, Md Shabaz Akhtar, Prakash Sharma · 2026
Read Comparative evaluation of four reference genes for qRT-PCR assays in snow trout (Schizothorax richardsonii) under multiple experimental conditions by Manchi Rajesh, Biju Sam Kamalam, Rini Joshi, Ciji Alexander, Md Shabaz Akhtar, Prakash Sharma (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13370014.txt.
Description
Manchi Rajesh and co-authors rank four house gene picks for gene read tests in a cold water trout, in a study from 2026. A test is only as true as the house gene used to scale it, and the best pick shifts by tissue, age, and tank heat. The team scored the four picks under eight test states with four rank tools.
One pick held most steady across all sixteen tissues of the fish. A growth factor pick led in early fry stages and in muscle of both sexes. The classic actin pick led in muscle across age, feed, and heat spans. The last pick swung with cold and was judged unfit as a scale. Test reads of two muscle genes proved how a poor pick warps results.
Catalog record
| CREATOR | Manchi Rajesh, Biju Sam Kamalam, Rini Joshi, Ciji Alexander, Md Shabaz Akhtar, Prakash Sharma |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 56 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |