A designed fusion tag improves soluble expression of nanobody in E. coli
Xingyu Wang, Ali Raza, Zhiqing Tao, Guan Wang, Xu Zhang, Maili Liu, Lichun He · 2026
Read A designed fusion tag improves soluble expression of nanobody in E. coli by Xingyu Wang, Ali Raza, Zhiqing Tao, Guan Wang, Xu Zhang, Maili Liu, Lichun He (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13355726.txt.
Description
Nanobodies, tiny binding parts taken from camel-family antibodies, are prized for tests and drugs, but making them in bacteria often ends in clumps. Xingyu Wang and six co-authors design a tag to keep them soluble. Their 2026 work fuses the nanobody to a helper protein from the same bacterium, one that guides folding.
The helper carries its own export signal, so the fused part ships to the cell's outer zone, where folding help is at hand. Gel scans during cleaning showed far more of the product in soluble form. The team cleaned about ten milligrams of one nanobody aimed at a cancer marker, and four milligrams of a second one. The tag offers a simple route to higher yields of a hard class of proteins.
Catalog record
| CREATOR | Xingyu Wang, Ali Raza, Zhiqing Tao, Guan Wang, Xu Zhang, Maili Liu, Lichun He |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 35 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |