CRISPR/Cas9-mediated PHOX2B functional knock-out in IMR32 neuroblastoma cells impairs neuronal excitability through dysregulation of ion channels genes
Silvia Cardani, Martina Bertocchi, Erika Donà, Filippo Chiesa, Clara Cambria, Ana Lucia Cuadros Gamboa, Eleonora Piscitelli, Vladimir Rancic, Simon Gosgnach, Silvia Pagliardini, Flavia Antonucci, Diego Fornasari, Simona Di Lascio, Roberta Benfante · 2026
Read CRISPR/Cas9-mediated PHOX2B functional knock-out in IMR32 neuroblastoma cells impairs neuronal excitability through dysregulation of ion channels genes by Silvia Cardani, Martina Bertocchi, Erika Donà, Filippo Chiesa, Clara Cambria, Ana Lucia Cuadros Gamboa, Eleonora Piscitelli, Vladimir Rancic, Simon Gosgnach, Silvia Pagliardini, Flavia Antonucci, Diego Fornasari, Simona Di Lascio, Roberta Benfante (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13341513.txt.
Description
Knocking out a master gene in nerve cells reshapes their electrical behavior, in a 2026 study by Silvia Cardani and colleagues. PHOX2B directs autonomic nervous system development, and its mutations cause neuroblastoma and a syndrome of failed breathing control, yet its target genes remain partly unknown.
Using CRISPR in IMR32 neuroblastoma cells, the team combined expression, binding, electrical, and imaging analyses. The knockout cells matured more in form yet fired oddly, with shifted resting voltage and weaker calcium responses. PHOX2B proved to repress ion channel genes including KCNQ5, SCN3A, and RYR2 directly. Checks in stem-cell-derived neurons from patients supported the developmental relevance.
Catalog record
| CREATOR | Silvia Cardani, Martina Bertocchi, Erika Donà, Filippo Chiesa, Clara Cambria, Ana Lucia Cuadros Gamboa, Eleonora Piscitelli, Vladimir Rancic, Simon Gosgnach, Silvia Pagliardini, Flavia Antonucci, Diego Fornasari, Simona Di Lascio, Roberta Benfante |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 75216 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |