An effective enzyme-linked immunosorbent assay-based method for quantification of gibberellic acid in pecan (Carya illinoinensis)

Mohadeseh Jahanifard, Ming Yang, Amandeep Kaur, Lu Zhang · 2026

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Description

Measuring a plant hormone in tough pecan tissue is made practical in a 2026 methods study by Mohadeseh Jahanifard and colleagues. ELISA testing for gibberellic acid is cheap and reliable, but hard, metabolite-rich pecan buds and bark resist standard handling.

Trials varied sample amounts, tissue-to-methanol ratios, and drying methods. Using about 0.0125 g of heat-dried or freeze-dried tissue at a low solvent ratio gave consistently strong readings, and hormone levels stayed stable after long storage at −80 °C. Buds held more gibberellic acid than bark, and bark values predicted bud values well, which helps when bud material is scarce.

Catalog record

CREATORMohadeseh Jahanifard, Ming Yang, Amandeep Kaur, Lu Zhang
YEAR2026
COLLECTIONPAPERS
FORMATTXT
FILE SIZE30591
LICENSEhttps://creativecommons.org/licenses/by/4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/article/MED/PMC13341426

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