Step-by-step En face O red oil method for aortic plaque staining and quantification in ApoE knockout mouse
Nurin Yasmin Mohd Khairudin, B. Vimala R.M.T Balasubramaniam, Suhaila Abd Muid, Nurdiyana Nasrudin, Nasibah Azme · 2026
Read Step-by-step En face O red oil method for aortic plaque staining and quantification in ApoE knockout mouse by Nurin Yasmin Mohd Khairudin, B. Vimala R.M.T Balasubramaniam, Suhaila Abd Muid, Nurdiyana Nasrudin, Nasibah Azme (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13330531.txt.
Description
Nurin Yasmin Mohd Khairudin and colleagues give a step-by-step guide to staining artery plaque in mice in a 2026 methods article. The en face Oil Red O method colours plaque along the opened aorta in ApoE knockout mice. Results vary between labs due to cutting, imaging, and counting habits, so a shared routine helps.
Steps run from aorta harvest and trimming through staining, mounting, photo capture, and counting in Adobe Photoshop. A standard digital camera suffices for imaging, the authors note. Each step carries photo guidance for new users. The routine aims to make plaque shares comparable across labs in early heart disease studies with mice.
Catalog record
| CREATOR | Nurin Yasmin Mohd Khairudin, B. Vimala R.M.T Balasubramaniam, Suhaila Abd Muid, Nurdiyana Nasrudin, Nasibah Azme |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39767 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |