A DNA-guided prokaryotic argonaute enables programmable RNA cleavage for sequencing and quality control of in vitro-transcribed RNAs
Lizhi Liu, Eric A Hunt, Eric J Wolf, Ivan R Corrêa, Erbay Yigit, Sebastian Grünberg · 2026
Read A DNA-guided prokaryotic argonaute enables programmable RNA cleavage for sequencing and quality control of in vitro-transcribed RNAs by Lizhi Liu, Eric A Hunt, Eric J Wolf, Ivan R Corrêa, Erbay Yigit, Sebastian Grünberg (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13330523.txt.
Description
Lizhi Liu and colleagues show new uses for a bacterial Argonaute enzyme in handling lab-made RNA in a 2026 study. Argonaute cutters can be guided to chosen sequences without the strict site rules that limit Cas enzymes. The team used a DNA-guided enzyme from Mucilaginibacter paludis that cuts RNA.
Uses shown include polishing the tail end of messenger RNA made in vitro. Paired with mass spectrometry, the cutter helped grade cap quality at the head end and tail length and makeup at the other end. One-pot cutting with many guides aided RNA sequencing, change detection, and fingerprint checks. The authors present the enzyme as a flexible quality tool for RNA production.
Catalog record
| CREATOR | Lizhi Liu, Eric A Hunt, Eric J Wolf, Ivan R Corrêa, Erbay Yigit, Sebastian Grünberg |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 50780 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |