Metabolic engineering of Pichia pastoris for sustainable production of 1,8-cineole from methanol
Cui Zhao, Tengfei Wang, Hongling Liu, Yanpo Li, Weiwei Wang, XiHui Wang · 2026
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Description
Cui Zhao and co-authors engineer yeast to brew a eucalyptus compound from methanol in a 2026 study. Methanol, makable from carbon dioxide, is a tempting factory feedstock, and 1,8-cineole is a valued scent and medicine ingredient. The team rebuilt Pichia pastoris step by step to route methanol carbon into the terpene.
Adding a cineole synthase, strengthening the mevalonate pathway, and muting a rival route gave strong early output, and reshaping methanol assimilation lifted yield further. Boosting two genes, ADE3 and SHM2, curbed formaldehyde harm and raised yield by 82.2 percent in that step, ending at 386.3 mg/L in a 5 L bioreactor run. The platform suits green making of other terpenes too.
Catalog record
| CREATOR | Cui Zhao, Tengfei Wang, Hongling Liu, Yanpo Li, Weiwei Wang, XiHui Wang |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 41397 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |