Radical diffusion, not lifetime, determines the range of peroxidase-based proximity labelling
Samarpita Sen, Daniel St Johnston · 2026
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Description
Samarpita Sen and Daniel St Johnston report a cautionary finding on a popular protein-mapping method in a 2026 study. Peroxidase-based proximity labelling tags nearby proteins with biotin, and users assume the tag spreads only about 20 nanometres. Working in fly follicle tissue, they marked proteins at the top, side, and base of cells.
Standard conditions gave a strong base signal even when the tagged protein sat at the top or side, because the reactive radicals travel many micrometres rather than dying out fast. The basement membrane soaked up radicals, acting as a sink. Lowering the label dose or shortening reaction times restored faithful maps, so the authors urge careful tuning before trusting spatial results.
Catalog record
| CREATOR | Samarpita Sen, Daniel St Johnston |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 52023 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |