Bacillus subtilis DnaB forms multiple protein–protein interactions essential for DNA replication initiation
Aurélie Guyet, Reyes Ruiz Campoy, Petra Manja, Frederic D Schramm, Simone Pelliciari, Stepan Fenyk, Yuanyuan Li, Charles Winterhalter, Aravindan Ilangovan, Heath Murray · 2026
Read Bacillus subtilis DnaB forms multiple protein–protein interactions essential for DNA replication initiation by Aurélie Guyet, Reyes Ruiz Campoy, Petra Manja, Frederic D Schramm, Simone Pelliciari, Stepan Fenyk, Yuanyuan Li, Charles Winterhalter, Aravindan Ilangovan, Heath Murray (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13326638.txt.
Description
Aurélie Guyet and team showed that the Bacillus subtilis motor loader DnaB acts as a hub for starting DNA replication. Their 2026 study tackles an unclear step. The master starter DnaA unwinds the origin, letting two copying motors load onto each DNA strand. How loading is managed at the origin was still unknown. The team mapped DnaB parts key for the start, using single-particle cryo-imaging, AlphaFold3 predictions, and two-hybrid interaction analyses.
Three necessary protein-protein interactions emerged, linking DnaB to the start proteins DnaA, DnaD, and DnaI. The data suggest DnaB runs dual motor loading at the origin through these contacts. The authors propose a model for replication start in Bacillus subtilis and related Firmicutes pathogens that use DnaB-type loaders. It clarifies a conserved mechanism that could inform understanding of bacterial growth and its vulnerabilities.
Catalog record
| CREATOR | Aurélie Guyet, Reyes Ruiz Campoy, Petra Manja, Frederic D Schramm, Simone Pelliciari, Stepan Fenyk, Yuanyuan Li, Charles Winterhalter, Aravindan Ilangovan, Heath Murray |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 71 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |