Comprehensive Profiling of Human Urinary Mercapturic Acid Conjugates Associated with Exposure to Reactive Chemical Species Using Enzymatic Deacetylation and High-Resolution Mass Spectrometry
Yuan-Chih Chen, Man-Ni Zhuang, Jen-Yi Hsu, Yi-Chun Lin, Hsin-Yi Wu, Pao-Chi Liao · 2026
Read Comprehensive Profiling of Human Urinary Mercapturic Acid Conjugates Associated with Exposure to Reactive Chemical Species Using Enzymatic Deacetylation and High-Resolution Mass Spectrometry by Yuan-Chih Chen, Man-Ni Zhuang, Jen-Yi Hsu, Yi-Chun Lin, Hsin-Yi Wu, Pao-Chi Liao (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13325442.txt.
Description
Yuan-Chih Chen and colleagues build a wider net for urine markers of toxic chemical exposure in a 2026 study. Mercapturic acid conjugates form when the body detoxifies reactive compounds, but standard screening misses many of them.
A new workflow pairs enzyme deacetylation with high-resolution mass spectrometry and a home-built library of 734,170 possible structures. Spiked tests on 11 standards validated the method. Applied to urine from 15 people before and after eating deep-fried food, it mapped 847 features to candidate structures, far above the roughly 100 in earlier work. After the meal, 59 markers rose and 11 fell, pointing to shifts in aldehyde handling.
Catalog record
| CREATOR | Yuan-Chih Chen, Man-Ni Zhuang, Jen-Yi Hsu, Yi-Chun Lin, Hsin-Yi Wu, Pao-Chi Liao |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 60354 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |