In vitro generation of human embryonic stem cell-derived heart organoids possessing physiological ion currents
Jiyoung Lee, Hiroshi Matsukawa, Kohei Sawada, Rin Kaneko, Fumitoshi Ishino · 2026
Read In vitro generation of human embryonic stem cell-derived heart organoids possessing physiological ion currents by Jiyoung Lee, Hiroshi Matsukawa, Kohei Sawada, Rin Kaneko, Fumitoshi Ishino (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13324305.txt.
Description
Jiyoung Lee and co-authors grow human heart organoids with working electrical currents, in a 2026 stem cell study. Their culture recipe for embryonic stem cell derived organoids used concentrated laminin-entactin and growth factor FGF4, plus BMP4, BMP10 and a ROCK blocker to steer heart formation.
The organoids formed atrium-like and ventricle-like chambers holding heart muscle, smooth muscle and vessel lining cells. They carried gap junction proteins and the main cardiac ion channel proteins, and they contracted as whole structures. Single heart cells taken from them showed sodium and calcium currents under patch clamp testing, matching living heart behaviour in part. The authors offer the system for development studies and drug safety testing.
Catalog record
| CREATOR | Jiyoung Lee, Hiroshi Matsukawa, Kohei Sawada, Rin Kaneko, Fumitoshi Ishino |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 49809 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |