Reported Cryptococcus neoformans heat-killing protocols show incomplete killing
Binyi An, Yingjun Gao, Yingying Wu, Yubing Wu, Xiaoyu Wang, Qingtao Kong, Hong Sang, Haibo Liu · 2026
Read Reported Cryptococcus neoformans heat-killing protocols show incomplete killing by Binyi An, Yingjun Gao, Yingying Wu, Yubing Wu, Xiaoyu Wang, Qingtao Kong, Hong Sang, Haibo Liu (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13322030.txt.
Description
Binyi An and co-authors test whether published heat killing methods for a fungus truly work in a 2026 study. Heat killing of Cryptococcus neoformans is common in immune research and vaccine work. The team reviewed 50 papers and found 23 distinct protocols varying in heat, time, cell density, and sample volume.
Using two virulent strains, only 9 protocols killed every fungus, while 14 left survivors. Just 34 percent of papers reported complete killing, and only 20 described plating checks, so failures may often pass unseen. Sample volume and cell density strongly shaped results. The authors give evidence based guidance for safer, more reliable heat killing.
Catalog record
| CREATOR | Binyi An, Yingjun Gao, Yingying Wu, Yubing Wu, Xiaoyu Wang, Qingtao Kong, Hong Sang, Haibo Liu |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 16013 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |