10 × Genomics Flex Gene Expression is a powerful tool for single-cell transcriptomics of xenografts models
Oriol Llorà-Batlle, Anca Farcas, Doreth Fransen, Nicolas Floc’h, Sara Talbot, Alix Schwiening, Laura Bojko, John Calver, Natasa Josipovic, Kanstantsin Lashuk, Julia Schueler, Andrei Prodan, Dylan Mooijman, Ultan McDermott, the PERSIST-SEQ Consortium · 2026
Read 10 × Genomics Flex Gene Expression is a powerful tool for single-cell transcriptomics of xenografts models by Oriol Llorà-Batlle, Anca Farcas, Doreth Fransen, Nicolas Floc’h, Sara Talbot, Alix Schwiening, Laura Bojko, John Calver, Natasa Josipovic, Kanstantsin Lashuk, Julia Schueler, Andrei Prodan, Dylan Mooijman, Ultan McDermott, the PERSIST-SEQ Consortium (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-13321738.txt.
Description
Llorà-Batlle and co-authors show that a probe-based gene profiling kit built for single human or mouse samples also works on xenografts, where human tumor cells grow inside a mouse. The kit reads fixed or frozen tissue, easing handling, but its probe sets exist only for the two species separately. The team ran both probe sets together in one reaction on mixed samples.
The standard software then produced separate count tables per species, but some probes reacted across species and blurred the split. Their cleanup step removes those cross signals, leaving clean tables for each species. The approach profiles graft and host tissue together with no cell sorting first, opening host-response studies in tumor models.
Catalog record
| CREATOR | Oriol Llorà-Batlle, Anca Farcas, Doreth Fransen, Nicolas Floc’h, Sara Talbot, Alix Schwiening, Laura Bojko, John Calver, Natasa Josipovic, Kanstantsin Lashuk, Julia Schueler, Andrei Prodan, Dylan Mooijman, Ultan McDermott, the PERSIST-SEQ Consortium |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 42 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |