Generating drug resistance models in human and murine cancer cell lines and assessing cross-resistance to chemotherapeutics and KRAS inhibitors
Nicole Martinez, Maria Fumagalli, Ravindra Gujar, Lev Becker, Chang Cui · 2026
Read Generating drug resistance models in human and murine cancer cell lines and assessing cross-resistance to chemotherapeutics and KRAS inhibitors by Nicole Martinez, Maria Fumagalli, Ravindra Gujar, Lev Becker, Chang Cui (2026) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/PMC13321002.txt.
Description
Nicole Martinez and co-authors present a 2026 laboratory protocol for building cancer cell lines that resist treatment. Resistance and cross-resistance shorten how long cancer drugs keep working, yet shared in vitro methods for growing resistant models are scarce. The protocol covers human and mouse cell lines exposed to chemotherapy drugs and KRAS blockers.
Steps guide readers through growing, expanding, and keeping resistant cells under drug pressure. Later steps check that resistance is real, test whether it spills over to other drugs, and run a measured cell killing assay. The aim is a repeatable system for judging the resistance risks of new compounds before they move toward patients.
Catalog record
| CREATOR | Nicole Martinez, Maria Fumagalli, Ravindra Gujar, Lev Becker, Chang Cui |
|---|---|
| YEAR | 2026 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 108397 |
| LICENSE | https://creativecommons.org/licenses/by/4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |